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How temperature affects enzymes

Activity rises to an optimum, then falls fast as the enzyme denatures.

The common mistake

Saying enzymes 'die' at high temperatures, or that they work faster forever as it heats up.

Instead: Heat speeds collisions up to the optimum. Past it, the active site changes shape (denatures) and the substrate no longer fits.

Worked example with Guide me

Why does a human enzyme stop working at 60°C?

  1. Sugar · What's the optimum for most human enzymes?
    Student · About 37°C.
  2. Sugar · What happens to the active site at 60°C?
    Student · It changes shape.

Answer: It denatures — the substrate no longer fits the active site.

Try 3 practice questions

  1. 1. What does 'denature' mean?

    Show answer

    The active site permanently changes shape

  2. 2. What else changes enzyme activity besides temperature?

    Show answer

    pH (also substrate concentration)

  3. 3. Are enzymes used up in a reaction?

    Show answer

    No, they can be reused

Quick answers

What is the most common mistake with how temperature affects enzymes?
Saying enzymes 'die' at high temperatures, or that they work faster forever as it heats up.
How do I get how temperature affects enzymes right?
Heat speeds collisions up to the optimum. Past it, the active site changes shape (denatures) and the substrate no longer fits.
Can you show a worked example?
Why does a human enzyme stop working at 60°C? It denatures — the substrate no longer fits the active site.

Related concepts

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